Molecular detection of viable bacterial pathogens in water by ratiometric pre-rRNA analysis.
نویسندگان
چکیده
Ratiometric pre-rRNA analysis (RPA) detects the replenishment of rRNA precursors that occurs rapidly upon nutritional stimulation of bacterial cells. In contrast to DNA detection by PCR, RPA distinguishes viable from inactivated bacteria. It exhibits promise as a molecular viability test for pathogens in water and other environmental samples.
منابع مشابه
Molecular Viability Testing of Bacterial Pathogens from a Complex Human Sample Matrix
Assays for bacterial ribosomal RNA precursors (pre-rRNA) have been shown to distinguish viable from inactivated bacterial cells in drinking water samples. Because the synthesis of pre-rRNA is rapidly induced by nutritional stimulation, viable bacteria can be distinguished from inactivated cells and free nucleic acids by measuring the production of species-specific pre-rRNA in samples that have ...
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عنوان ژورنال:
- Applied and environmental microbiology
دوره 76 3 شماره
صفحات -
تاریخ انتشار 2010